Daily Archives: December 12, 2017

Assaying protein-protein interaction by the complementation of firefly luciferase catalytic steps

Protein – protein interaction (PPI) assay is a key technology for various fields from basic molecular biology to diagnosis and drug discovery. Previously, PPI assays based on fluorescence resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET)

Nuclear shuttling of key methionine cycle enzymes in acute liver injury

Important metabolic enzymes have been classically ascribed to certain subcellular locations, where they perform their well-known function. In the last decades, several technological advances have allowed detection of these proteins in new locations, performing either their known