A new extracellular role for miR-939 in triple negative breast cancer
Triple negative breast cancer (TNBC) is defined as estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) negative breast carcinomas (BC). Unfortunately, this kind of tumor is characterized by poor prognosis, high rates of early local relapse and distant metastasis.
Due to the aggressiveness of TNBC, we focused our research on the mechanisms underlying the ability of TNBC cells to leave the primary tumor, enter in the blood stream and spread to distant organs to form secondary foci. In particular in this paper we studied the role of miR-939 in TNBC biology. MicroRNA (miRNAs or miRs) are small-non coding endogenous RNA molecules involved in the control of gene expression and their deregulation was associated with the risk and/or survival in various malignancies like breast cancer.
By analyzing The Cancer Genome Atlas data base that collects information from more than 1000 BC, we observed that TNBC expresses higher levels of miR-939 in comparison with other BC subtypes. Furthermore, the results obtained from our cohort of 63 TNBC patients from the Istituto Nazionale dei Tumori of Milan suggest that miR-939 represents a negative prognostic factor for disease free survival. Indeed, lymph node-positive tumors overexpressing miR-939 have an increased risk of relapse as compared with those with lower levels of miR-939 and lacking of lymph node involvement. During the formation of metastases, cancer cells acquire the ability to traverse the blood vessels to favor tumor invasion. In particular, cancer cells can release microvesicles (i.e. exosomes) to alter vessel permeability by the transfer of protein, soluble factors, RNAs, and miRNAs in endothelial cells. These cells express adhesion molecules like cadherin 5 (VE-cadherin) and zonula occludens (ZO-1) to form respectively adherens and tight junctions on the cell membrane, the loss of which has been shown to favor the migration of invasive cancer cells. We demonstrated in vitro a direct regulation of VE-cadherin by miR-939. Moreover, we found that TNBC cells actively release exosomes containing miR-939 that are taken up by endothelial cells and, once internalized, causes a decrease of VE-cadherin expression inducing the loss of cell-to-cell contact and the disruption of endothelial barrier that favor transmigration of cancer cells.
Since the secretion of miR-939 is sufficient to open gates between cells in tumor blood and lymphatic vessels that express VE-cadherin on their surface, we can speculate that miR-939 plays an important role in cancer dissemination and progression.
Di Modica Martina, Casalini Patrizia, Elda Tagliabue, Triulzi Tiziana
Molecular Targeting Unit, Department of Research Epidemiology and Molecular Medicine,
Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy
Breast cancer-secreted miR-939 downregulates VE-cadherin and destroys the barrier function of endothelial monolayers.
Di Modica M, Regondi V, Sandri M, Iorio MV, Zanetti A, Tagliabue E, Casalini P, Triulzi T
Cancer Lett. 2017 Jan 1
|Can hypertension and related cardiovascular diseases… Hypertension is a common but one of the most important health problems, because it is a major risk factor for many cardiovascular diseases. Hypertension is a multi-factorial disease involving complex…|
|Melanoma is controlling metastasis of melanoma… The title is provocative, because most will most ask: but who other than mm can control the metastasis of melanoma? But let's get on the path of facts and procedures…|
|Lessons learned from the study of non-cancerous… Meningiomas are the most common among tumors inside the skull (35.6%). The cellular origin of these tumors is the membrane that surrounds the brain, the meninges (Fig. 1) Most meningiomas…|
|An introduction to modelling lymphocyte migration in vitro Lymphocyte recruitment to inflamed endothelial cells (EC) is fundamental to immune homeostasis - providing both cell-mediated defence against disease, while also contributing to the resolution of inflammation. However, the dynamic…|
|Structure and function of the esophageal mucosa Structure and function go hand-in-hand amongst the morphology, morphometry, biomechanics and secretory potential within the alimentary tract mucosa, submucosa and muscularis propria. Structure must evolve first during the development of…|
|Milk exosomes for drug delivery The need to effectively deliver drugs to target site has employed a multitude of substances varying from biological to a chemical nature and solid metals for the preparation of tiny…|