Fabrication of magnetic activated carbon as a new adsorbent for ultrasonic assisted magnetic solid phase dispersive extraction of bisphenol A from milk

Bisphenol A (BPA), is an organic chemical compound that has been widely used as a monomer in the manufacturing of polycarbonate plastics and epoxy resins, in thermal paper production and as an additive in the formation of some polyvinyl chloride plastics. BPA’ production is approximately 5 – 6 billion pounds annually.

Graphical Abstract.

The reason that BPA is of high concern is that it can act as a potential endocrine disruptor which imitates the action of estrogen hormone. The latter regulates reproduction and development. The activity of BPA is by binding to estrogen disruptors thus interfering with the action of endogenous gonadal steroid hormones leading to abnormal differentiation of reproductive organs.

How can BPA reach the human body? Food and beverage have been identified as the major sources of BPA exposure in humans as it may migrate from interior can coating and polycarbonate containers into the food or liquids due to acidic conditions or thermal treatment in processing.

Due to its high potential health risk, regulatory authorities have taken into action several limits, as for example the European Union (EU) which has set a limit of migration at 600 μg kg-1 for BPA in food. Moreover European Directive 2011/8/EU, prohibits within the EU the manufacture of polycarbonate infant-feeding bottles containing BPA, and prevents the importation of such products. The United States Food and Drug Administration (FDA) has banned the use of BPA in baby bottles and sippy cups since 2012. Recently, in 2015 the European Food Safety Authority (EFSA) has established a Tolerable Daily Intake (TDI) of 4 micrograms/kilogram body weight (µg/kg bw).

Apparently, the determination of BPA in several matrices including milk and human breast milk is of utmost importance. Several analytical techniques have been introduced for this purpose e.g. High Performance Liquid Chromatography (HPLC) with fluorescence (FLD) or ultraviolet (UV) detection, Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Gas chromatography coupled with mass spectrometry (GC-MS), Capillary electrophoresis (CE) and Amperometric determination.

Usually sample preparation techniques for the extraction of BPA from milk include solid phase extraction (SPE), matrix solid phase dispersion (MSPD), solid phase microextraction (SPME), microwave assisted ionic liquid microextraction (MAILME), stir bar sorptive extraction (SBSE), hollow fiber-liquid phase microextraction (HF-LPME) and molecularly imprinted SPE (MISPE).

Magnetic solid phase extraction (MSPE) is a sample preparation approach for the separation of analytes of interest from the sample solution by using magnetic sorbents and an external magnetic field. The magnetic sorbent is added to the sample solution, so that target analytes are adsorbed and then the magnetic sorbent is separated from the sample solution by applying an external magnetic field. After elution with an appropriate solvent, the extract is analyzed. In comparison with traditional SPE, MSPE has many obvious benefits including such as reduction of the time taken for analysis, low consumption of organic solvents, and easy handling.

The choice of an appropriate sorbent material is very crucial for the efficient extraction of BPA. Activated carbon has been proved as an effective adsorbent for the separation and purification of gaseous and liquid phase mixtures due to its outstanding characteristics e.g. high surface area, wide range of surface functional groups and internal microporosity. Magnetic activated carbons are composite materials consisted of non- magnetic activated carbon and magnetic labels, such as magnetite (Fe3O4) and magnemite (g-Fe2O3) providing the magnetic behavior of the composite.

In our study, the impregnation of a micro – mesoporous activated carbon with magnetite nanoparticles (Fe3O4) was effectively accomplished by sonication. The prepared magnetic activated carbon was characterized and the results proved that it had the desired magnetic susceptibility and high adsorption capacity for BPA. This material was used, for the first time, as adsorbent for ultrasonic assisted magnetic solid phase extraction of BPA from cow milk and human breast milk samples for subsequent determination by HPLC- UV. The developed method was sensitive, simple, low cost for operation and environmentally friendly.

Eleni A. Deliyanni, Victoria F. Samanidou
Department of Chemistry, Aristotle University of Thessaloniki, Greece

 

Publication

Fabrication and evaluation of magnetic activated carbon as adsorbent for ultrasonic assisted magnetic solid phase dispersive extraction of bisphenol A from milk prior to high performance liquid chromatographic analysis with ultraviolet detection.
Filippou O, Deliyanni EA, Samanidou VF
J Chromatogr A. 2017 Jan 6

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