Tag Archives: mRNA

mRNA Interactome Capture: a frontier method to discover mRNA-bound proteomes in plants

Cells are the structural and functional basic units of a given organism to support its life. The phenotype of an organism is highly determined by proteins at the molecular level in each cell. Imbalance of gene expression

Mother or father obesity may lead to offspring obesity in the adult life

The process by which early life factors influence offspring health in adulthood is defined as ‘fetal programming’ and is considered a key mechanism for the establishment of chronic diseases in the offspring adulthood. Given the obesity pandemic,

mRNA decay activity in neurons regulates survival of worms at high temperatures

The ability of an organism to quickly alter or fine-tune its gene expression profile as a response to unfavorable and stressful conditions is a vital trait that can determine its chances of growth and survival in unstable

Coordination of DNA transcription and DNA replication by the DNA damage response through the Ddx19 RNA helicase

DNA transcription is a fundamental cellular process that allows gene expression through generation of messenger RNA molecules (mRNA) that are then translated into proteins in the cytoplasm of the cell. However when cells proliferate, another key cellular

Early plant development under stress relies on RNA splicing

Plants are unable to move away from harmful environmental conditions that affect their well-being and that ultimately compromise their growth and thereby crop production. To cope with a constantly changing environment, they have evolved unique and elaborate

The proteins and their mRNAs may be located in different areas of the embryos

All proteins are synthesized in a cell in polysomes (polyribosomes) on the template (mRNA). That is why in molecular embryology, by tacit consent, a presence or absence of the specific mRNA in the cell indicates the presence

Serotonin and sleep regulation: cellular and molecular mechanisms

Serotonin ( 5-HT ) is a neurotransmitter, which plays major roles in several physiological functions including circadian rhythmicity, appetite, thermoregulation, nociception, emotion and cognition. The relationship between 5-HT and sleep was demonstrated by several experiments, including in particular an

Lessons learned from the study of non-cancerous meningioma tumors

Meningiomas are the most common among tumors inside the skull (35.6%). The cellular origin of these tumors is the membrane that surrounds the brain, the meninges (Fig. 1) Most meningiomas are grades I & II according to

A “selfish ribosome” as the origin of cellular life

Could there have been a form of “life” that integrated genetic information, protein translation, and the molecular machinery necessary to replicate itself before the first cells? Might such an entity still exist within our cells as a

MicroRNAs fighting muscle wasting during ageing

Have you ever wondered why we age? With time, our joints, bones and muscles become weaker; wrinkles appear and we lose hair and sense of hearing and sight. However, not only our body experiences external changes; inside,

Uncovering the microRNA landscape in the crop pest nematode Meloidogyne incognita

Plant-parasitic nematodes are small roundworms that predominantly feed on roots and cause crop yield losses. The root-knot nematode, Meloidogyne incognita is among the most damaging pests of several crops including cotton, tomato, and soybean. The infective stage

Silencing a microRNA may keep the doctor away for diabetic wounds

Patients with diabetes are frequently afflicted with impaired wound healing that may progress into chronic, diabetic ulcers, often leading to complications including limb amputation with increased risk of morbidity and mortality. These ulcers occur not only in

Certain transcripts synthesized by RNA polymerase III in mammals became polyadenylation-competent

It has been known for 40 years that eukaryotic mRNA molecules contain a region of up to 250 adenine residues at the 3’ end. This poly(A) tail mediates mRNA nuclear export, translation, and protection from degradation in

A new technique for quantification of small RNA variants with a single-nucleotide resolution

Non – protein – coding regions of the genome are widely transcribed to produce non – coding RNAs (ncRNAs), which play a crucial role in various biological processes and diseases. Recent advances in next-generation sequencing technologies have

Neurodegeneration-associated RNA-binding protein, FUS, regulates mRNA length

RNA processing, including alternative regulations of transcription initiation, splicing, and 3’ end formation of pre – mRNA, is a highly specialized mechanism that enables to generate multiple isoforms from a single gene. This mechanism expands transcriptome and

Going my way? A tale of enzyme recruitment and activation

Protein synthesis is an energetically costly cellular process, in part due to the metabolic requirements of ribosome assembly and gene-specific messenger RNA (mRNA) production.  Consequently, biosynthetic flux through the protein translation pathway is tightly regulated while translational