Osteoarthritis alleviation by rewiring cellular energy metabolism
Osteoarthritis (OA) is a chronic disorder associated with degeneration of the joint, including articular cartilage, subchondral bone, synovial tissue, tendons, periarticular muscles that involves low-grade systemic inflammation. Recent studies have demonstrated the involvement of chondrocyte differentiation (hypertrophy) as one of the mechanisms in cartilage degradation in OA. This finding suggests common regulatory mechanisms that govern chondrocyte differentiation and articular cartilage destruction in OA. As cell proliferation and differentiation are regulated by the cellular metabolic status related to energy production, here we suggest that knowledge of the mechanisms required for maintenance of energy homeostasis could provide a means to reveal the primary causes of OA development.
Eukaryotic cells can generate energy in the form of ATP either using the anaerobic process of glycolysis producing two ATP molecules or by aerobic oxidative phosphorylation (OXPHOS) generating up to 30 ATP molecules. The OXPHOS system integrates the production of cellular energy with major catabolic pathways, including the tricarboxylic acid (TCA) cycle and fatty acid or amino acid oxidation. It contains five multimeric mitochondrial proteins: Complexes I-V. The chain of redox reactions produces an electrochemical gradient, which is used by Complex V to drive ATP synthesis.
Intracellular regulation of energy production is principally important for differentiation during normal growth. Multiple signalling pathways affect both proliferation and differentiation and control cellular energy metabolism. Anabolic signalling pathways such as mammalian target of rapamycin (mTOR) mostly stimulate glycolysis. AMP-activated protein kinase (AMPK) promotes catabolic activity and fatty acid oxidation, inhibits glycolysis, and upregulates the TCA cycle.
Endochondral ossification (Fig. 1), the mechanism responsible for the development of long bones, is dependent on an extremely stringent coordination between the processes of chondrocyte maturation in the growth plate, vascular expansion in the surrounding tissues, osteoblast differentiation, and osteogenesis. Growth plate cartilage is a highly metabolic but poorly vascularized tissue adapted to generating energy primarily by glycolysis. However, the increase in the mitochondrial protein fraction and the decrease in mitochondrial volume observed in resting cartilage compared to the hypertrophic zone indicates that residing chondrocytes change their energy status during endochondral ossification.
In OA (Fig. 1), inflammatory mediators induce mitochondrial dysfunction and increased production of reactive oxygen species. Oxidative stress might also damage the chondrocyte mitochondrial respiratory chain, as evidenced by the decreased activity of Complexes I, II, and III, causing decreased mitochondrial ATP generation. Increased glycolysis activity suggests that OA chondrocytes from anti-inflammatory reliance on oxidative phosphorylation and the TCA cycle switch to proinflammatory reliance on glycolysis for energy.
In addition, the metabolic syndrome components such as abdominal obesity, hypertension, hyperglycaemia, significantly increase the risk of disturbances to normal functioning of energy and nutrient sensors in articular cartilage. At the molecular level, metabolic dysregulation in OA was associated with reduced activities of AMPK as well as increased mTOR expression in articular chondrocytes.
Furthermore, reduced AMPK gene expression in OA articular cartilage was accompanied by its increased expression in the peripheral blood cells from the same OA patients. This suggests that whole-body energy availability and that in articular cartilage can differ in OA patients and cause an energy appeal reaction, a process that involves the redirection of energy-rich fuels from energy sources, such as articular cartilage in OA for further energy relocation to other specific body sites in the course of its destruction. Indeed, the upregulation of collagen type II expression in OA cartilage with concomitant upregulation of TCA-related gene expressions points to the potential for availability of energy-generating substrates required for matrix repair by chondrocytes in OA patients.
Nasonova Research Institute of Rheumatology, Moscow, Russia
Regulation of energy metabolism in the growth plate and osteoarthritic chondrocytes.
Tchetina EV, Markova GA
Rheumatol Int. 2018 Nov
|Cellular stress and AMPK links metformin and jumping… The evolution of the human genome has been facilitated to a great extent by the activity of transposable elements (TEs), also known as “jumping genes”. As the name implies, TEs…|
|Large animal models for osteochondral regeneration Due to the lacking of a blood supply and the low cellular density of articular cartilage, this hard tissue has a limited healing capacity. Therefore, focal traumatic events causing cartilage…|
|Why are skeletal muscles not depleted of energy… ATP is a universal carrier of energy in a form easily accessible for different reactions in the cell. Its hydrolysis to ADP and Pi (inorganic phosphate) drives processes that cannot…|
|Look ma, no air Until recently, it has been conventional thinking that mammals cannot live without oxygen. This has been challenged by studies demonstrating survival of mole rats without oxygen for extended periods of…|
|Cellular stress and AMPK activators including… The neural mechanisms that give rise to human consciousness have been described as one of the greatest and most profound mysteries in all of modern medicine. The use of general…|
|Mitochondrial Complex I activity signals antioxidant… The vast majority of eukaryotic cells perform oxidative phosphorylation (OXPHOS), which uses the energy released by the mitochondrial oxidation of certain metabolites, i.e. glucose, to produce adenosine triphosphate (ATP). OXPHOS…|