Research on microplastic effects in aquatic environments has expanded dramatically in the last decade. However, many important questions remain largely unanswered. We still do not know, for example, what are the safe levels of microplastic in different ecosystems. This kind of information is vital for the regulation of the microplastic as a pollutant. What makes this situation even more challenging is that we have a very unsettled methodology to evaluate the toxicity of solid waste, such as plastic litter.

Fig. 1.

We presented a novel approach for testing the hazard potential of solid waste particles, including microplastic. Natural lake or seawater always contain small particles, both mineral and organic, in the same size range as the microplastic (Fig. 1A). However, relative to the amount of these particles, the quantities of microplastic are low. At high levels, the naturally occurring particles, such as clay, can be harmful to various animals, from water fleas to fish, in ways similar to microplastic. In very turbid water, solid particles may hamper breathing and feeding of these animals by clogging their gills and guts.

To determine safe levels of microplastic, we must find out what contribution of plastic to the total amount of particles in the environment that causes no harm. Using this concept, we developed a method to estimate the effects caused by microplastic in a mixture with natural particles, i.e., the real-life exposure scenario.

The first step of the test is to identify the hazardous level of all particles in the mixture. Here, the test system can be adjusted to mimic turbidity levels in different waters (i.e., from a clear water lake to turbid estuaries and rivers) and take into account seasonal and human-induced variability. The second step is to determine how much microplastic is safe in that environment and provide threshold values that are easy to understand and set as acceptable in environmental regulations.

We tested our approach experimentally using water fleas exposed to a mixture of clay and polyethylene terephthalate (PET). These animals filter their food (e.g., algae) from the water and are, thus, naturally exposed to suspended solids (particles). In the experiment, we manipulated both the amount of total suspended solids (i.e., clay + PET) and PET proportion in the mixture (Figure 1B). The water fleas survival (Fig. 1C) provided two threshold values. I) what total suspended solid levels they can tolerate and II) the safe level of PET in this mixture (Fig. 1D).

We found that water fleas can tolerate up to 24 mg per L of suspended solids, which is a relatively high concentration typical for turbid waters. When the PET proportion in the suspension exceeded 2.4%, the mixture became more hazardous than that composed by the pure clay. These levels of microplastic are very high compared to the concentrations reported so far from lakes and seas.

To disentangle responses to microplastic exposure from those to other suspended solids, both microplastic and natural particles must be present in the test system. We believe that more ecologically relevant test methods are needed for understanding how and when microplastic becomes a hazard.

Zandra Gerdes, Elena Gorokhova
Department of Environmental Science, Stockholm University, Stockholm, Sweden

Publication

A novel method for assessing microplastic effect in suspension through mixing test and reference materials
Gerdes Z, Hermann M, Ogonowski M, Gorokhova E
Sci Rep. 2019 Jul 23

Read offline:     

Related Articles:

	When toxicity goes circular: Isoprostanes as markers and… In normal conditions, there is a dynamic equilibrium between oxidants and antioxidants in the human body; however, when there is an unbalance in this redox state, oxidative stress occurs.  In…
	Actinobacteria from Algerian ecosystems as a rich source of… Secondary metabolites are molecules not essential for the growth and development of microorganism, mainly having a defence role. They have shown a great potential for the discovery of drugs and…
	Reuse of third generation industrial waste As global consumption of finite raw materials continues to rise, so too does the amount of waste produced. In Europe for example, each citizen consumes an average of 15 tonnes…
	Invasion of science and nature by the parthenogenetic… The marbled crayfish or Marmorkrebs, Procambarus virginalis (Fig. 1A), is the only obligately parthenogenetic decapod crustacean. It was detected in 1995 in the German aquarium trade. With almost 300 publications,…
	Epigenetic phenotype diversification helps animals to adapt… There is increasing evidence that epigenetic phenotype variation can contribute to environmental adaptation, speciation and evolution. Epigenetic changes of the phenotype are caused by differences in gene expression rather than…
	May polystyrene microplastics be ingested by marine… World plastic production has increased steadily over the past 50 years, reaching over 350 million tonnes in 2018. Most of the plastic waste ends up in the oceans and it…