Quality assessment of marketed tea products
Chamomile tea composed of dried flower heads of Matricaria recutita L. (Asteraceae) is one of the most popular single ingredient herbal teas. Tea industries, spice shops or public bazaars are mostly supplied with chamomile as a raw material via cultivation or through nature-picking. However, one of the drawbacks of nature-picking is adulteration. Therefore, quality control of raw chamomile materials before marketing should be carefully considered not only by quantification of apigenin 7-O-glucoside (A7G, active marker) but also by fingerprinting of chemical composition.
This work presents both quantification of A7G and chemical fingerprinting of commercial chamomile tea products (tea bags and crude chamomile flowers) obtained from different food stores and spice shops by a validated HPTLC method. HPTLC chromatogram comparison of marketed chamomile tea products indicated that not chamomile tea bags but crude flowers sold on market were adulterated with other plant materials (Fig. 1). A7G content determined by HPTLC method in different packed tea brands was ranged from 0.43 to 0.80 mg/g, while in most of the crude chamomile samples sold in spice shops, A7G was either not determined or was below the limit of quantification (LOQ), which is 5 ng/band. Eventually, crude flowers sold as chamomile in spice shops would not met with therapeutic expectations of people.
In addition, HPTLC profiles of investigated chamomile tea samples were compared with HPLC method stated in the European Pharmacopoeia (Ph. Eur.). In the Ph. Eur. instead of free A7G content, total A7G amount (including mono- or diacetylated derivatives of A7G) is estimated in hydroalcoholic chamomile extract. Powdered chamomile flowers extracted with ethanol was subjected to HPTLC analysis both directly and following the ester hydrolysis by addition sodium hydroxide reagent to the extract. Acording to the HPTLC chromatogram many components disappeared after addition of sodium hydroxide. In other words, hydrolysis caused to eliminate many other compounds which may help both to discriminate the genuine specimen and evaluate the possible adulteration. It should be underlined that although A7G is an active marker, it should not be considered as a chemotaxonomic marker for chamomile authentication.
In addition, HPTLC fingerprint profiles combined with pattern recognition techniques of these marketed products were comparatively evaluated with wild and cultivar chamomile samples and also chamomile-like species from Asteraceae. Blue and green channels (Fig. 2) showed well separation and provided good discrimination among samples. In order to search for natural groupings among the samples, cluster analysis was applied on data set. According to blue channel, dendrogram shows three main clusters. First cluster consisted of different brands of chamomile tea bags, wild chamomiles, Bodegold and one Bona type cultivars show similar profile. Second cluster consisted of crude flowers from spice shops grouped with Anthemis sp., Tanacetum sp. and Chrysanthemum sp., indicating their similar chemical profile resulted in either potential adulteration or misidentification. Bona and Zloyt Lan varieties of chamomile cultivars and one cultivar from Yalova formed a third cluster.
In case of green channel, first principal component PC1 describes 38.48%, while PC2 19.69% of total variability. According to PCs score, crude flowers on market were overlapped with Anthemis sp., Tanacetum sp., and Chrysanthemum sp. indicating that probably crude flowers were adulterated with those species from Asteraceae. Chamomile tea bags from different brands were close to wild chamomile samples and Bellis sp., except several Anthemis spp. which formed separate cluster according to PC2. Chamomile cultivars were positioned on the upper right side indicating high dissimilarity from other Asteraceae samples.
Consequently, it is obvious that determination of not only the amount of active ingredient(s) but also ensuring genuine specimen plays a vital role for public health in order to avoid adverse reactions.
Etil Guzelmeric 1, Petar Ristivojević 2, Irena Vovk 3, Dušanka Milojković-Opsenica 4,
Erdem Yesilada 1
1Yeditepe University, Faculty of Pharmacy, Department of Pharmacognosy and Phytotherapy,
2Innovation Centre of Faculty of Chemistry Ltd., Belgrade, Serbia
3Department of Food Chemistry, National Institute of Chemistry, Ljubljana, Slovenia
4Faculty of Chemistry, University of Belgrade, Belgrade, Serbia
Quality assessment of marketed chamomile tea products by a validated HPTLC method combined with multivariate analysis.
Guzelmeric E, Ristivojević P, Vovk I, Milojković-Opsenica D, Yesilada E
J Pharm Biomed Anal. 2017 Jan 5
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