Tag Archives: cell culture

Making mountains out of molehills: how stem cells become platelets

Heamatopoietic stem cells purified from cord blood or bone

You have 100,000 km of plumbing (blood vessels) in your body. What then keeps them from leaking? This essential work is carried out by tiny cells called platelets, which are able to form a plug to stop

Unregulated oxygen levels in mammalian cell culture changes cell physiology

As aerobic organisms we continuously utilize oxygen from the surrounding environment which ultimately participates in the formation of useful bioenergetic intermediates. Approximately 21% of the air we breathe is composed of oxygen, but the quantity of oxygen

An introduction to modelling lymphocyte migration in vitro

Lymphocyte recruitment to inflamed endothelial cells (EC) is fundamental to immune homeostasis – providing both cell-mediated defence against disease, while also contributing to the resolution of inflammation. However, the dynamic relationship between lymphocytes and EC can become

Go with the flow: Studying the movement of T-cells to sites of inflammation

Inflammation is the protective response of the immune system that aids the removal of invading infectious agents and repair tissue injury. It involves the movement of white blood cells (leukocytes) from the bloodstream, through the cells lining

Use of an in vitro model of blastema tissue from Rabbit pinna

Rabbit ear wound repair is an accepted model for studies of tissue regeneration, leading to scar less wound repair. It is believed that a specific tissue, blastema, is responsible for such interesting capacity of tissue regeneration. To

Bone cells prefer to grow on aligned fibers

The finding of suitable biomaterials allowing the bone restoration of large bone defects is the subject of active research. Non-resorbable biomaterials such as polymers can constitute suitable scaffolds allowing the deposition of new bone. Microfibers made with

Single-cell-culture experiment made easier for researchers

Single-cell-culture experiment is widely used for biological researches concerning cell heterogeneity. This experiment although straightforward to performed by the limiting dilution method with manual pipetting and cell culture well plates, is time consuming and not cost-effective. To

Development of anti-atherosclerotic drugs on the basis of cell models or how to make the elderly pay for placebos

The large research series has become internationally known in 1986 after a publication by the ex-Soviet minister of health as the first author and continues until today. In brief, cell cultures have been used for evaluation of

Transformative nanomaterial synthesis using biological conditions

One area of promise and concern when using nanomaterials is their potentially high level of reactivity. This comes from the massively increased surface area of the material when it is segmented into much smaller pieces. A copper

Sandwiches to better understand cells

The human body is formed by complex organ systems. These organ systems are moreover composed by cells of different types that have to co-operate in order to make the body work properly. This is the reason why