The ultimate treatment for end-stage renal disease is orthotopic transplantation. However, the demand for kidney transplantation far exceeds the number of available donor organs. While more than 100,000 Americans need a kidney, only 17,000 people receive a kidney transplant each year (National Kidney Foundation estimate).

In recent years, several regenerative medicine/tissue engineering approaches have been exploited to alleviate the kidney shortage crisis. Although cell-based therapy shows promise in experimental animal models, it cannot be applied when renal structure has been profoundly altered, as in chronically injured kidney. In these cases, kidney regeneration is needed to rebuild a whole functional kidney de novo, eliminating the need for dialysis.

To rebuild a kidney, some investigators have proposed emulating early stages of organogenesis by implanting the primordial kidney, also known as the metanephros.

For tissue transplantation to be effective, a hospitable site must be relatively accessible, provide sufficient space for cell expansion and offer access to vasculature for long-term engraftment support. In our search to find an ideal transplantation site, we found the lymph node to be a good candidate. Lymph nodes, secondary lymphoid organs, can be viewed as mini-bioreactors for T cells after immune activation. They are also hijacked by cancer cells during metastatis. We speculate that lymph nodes also provide the necessary regulatory cues to normal cells and transplanted tissues. Indeed, by directly injecting the lymph node with embryonic kidney tissues, we demonstrated engraftment of the donor cells and subsequent organ function. While there is room for further improvement regarding a contiguous collecting duct epithelium to create a single path for urine, the degree of graft function observed in our study supports the lymph node as a site for kidney tissue engineering. With the rapid advancement in the human induced pluripotent stem cell (iPSC) field, enabling the induction of pluripotency in mature somatic cells by treatment with defined factors, the lymph node platform also holds great promise in the generation of replacement kidneys from a patients’ own cells. Our future work will therefore encompass an integrated tissue engineering approach that combines a scaffold-free lymph node-based system implanted with human stem/progenitor cells to regenerate a functional kidney.

Renal progenitors can be obtained from patient-derived iPSCs (for example reprogrammed from dermal fibroblasts or kidney cells) following exposure to various combinations of nephrogenic factors. The progenitor populations that will likely be necessary for rebuilding a functional kidney are nephron progenitors (giving rise to all nephron epithelia), ureteric epithelium progenitors (giving rise to collecting duct cells), renal stromal cells progenitors (giving rise to pericytes, mesangial cells, vascular smooth muscle, and subsets of peritubular endothelium), and vascular progenitors (giving rise to glomerular and non-glomerular endothelium). Following culture of renal progenitors within a lymph node-based bioreactor, nephron morphogenesis is expected to occur, leading to de novo kidney organogenesis. Lymph nodes adjacent to kidneys could be transplanted and graft-host ureter connection achieved through surgical and/or engineering techniques.

Our study will offer a new approach for kidney disease treatment in a patient population with limited therapeutic options.

Maria Giovanna Francipane, Eric Lagasse
McGowan Institute for Regenerative Medicine, Department of Pathology,
University of Pittsburgh School of Medicine, Pittsburgh, PA, 15219, USA

Publication

The lymph node as a new site for kidney organogenesis.
Francipane MG, Lagasse E
Stem Cells Transl Med. 2015 Mar

Read offline:     

Related Articles:

	Systemic therapy in juvenile localized scleroderma Juvenile localized scleroderma, also known as morphea, is an rare condition, it occurs around 1 in 5000 children, where the skin and the tissues underneath the skin like fat tissue,…
	Improving assessment of arthritis models to better… Rheumatoid Arthritis (RA) is a common inflammatory disease that is characterized by swelling and tenderness of multiple joints. The resulting pain and joint stiffness cause disability for patients and treatment…
	UCLA researchers develop high-sensitivity… A significant advancement for point-of-care medical diagnostics, a team of researchers from UCLA has introduced a deep learning-enhanced, paper-based vertical flow assay (VFA) capable of detecting cardiac troponin I (cTnI)…
	UCLA researchers pioneer AI-based tissue staining to… Los Angeles, CA – September 10, 2024 – Researchers at the University of California, Los Angeles (UCLA) have pioneered a groundbreaking approach in the imaging and detection of amyloid deposits…
	Rabbits with mammary carcinomas as a model for… Within a breeding colony of rabbits, the American pathologist Harry Greene (1904-1969) observed that mammary carcinomas were restricted to certain families. This is suggestive of a familiar predisposition as it…
	Your pharmacist will see you now! Public perception of pharmacists might be limited to dispensing medications. However, pharmacists are trained to deliver patient care to help patients improve their health-related outcomes and achieve their goals. Recognizing…