Tag Archives: fluorescence
Method to measure calcium signaling in nanometer-sized primary cilia
Cilia are slender microtubule-based organelles that protrude from the apical membrane in most adherent cells. Studies looking into the mechanosensory function of the primary cilium have proved it to be an essential cellular organelle. When exposed to
Measuring the pH levels inside lysosomes
The pH level varies in different organelles inside a cell and there is a diversity of function carried out within. Any deviations of pH from normal levels could signal cellular stress or dysfunction and may indicate serious
Watching caveolae under stress
The plasma membrane of many cells contain many protein-dense invaginations called caveolae or ‘little caves’. Caveolae are composed of ~140 molecules of caveolin proteins along with other components. Caveolae are thought to act like springs and flatten
Assaying protein-protein interaction by the complementation of firefly luciferase catalytic steps
Protein – protein interaction (PPI) assay is a key technology for various fields from basic molecular biology to diagnosis and drug discovery. Previously, PPI assays based on fluorescence resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET)
A figure-of-eight molecule twists the red light
Development of fluorophores for CPL, circularly polarized luminescence, has attracted much attention. CPL has large potential toward future applications such as 3D displays and biosensing. Major challenges in creating good CPL fluorophores are; 1) large anisotropic dissymmetric
How to characterize best the genetic content of small supernumerary marker chromosomes
An inborn genetic defect can be found prenatally or postnatally. The best known and most frequently observed reason for such developmental delay and/or mental retardation is Down-syndrome, also known as trisomy 21. I.e. besides the normally present
Chronic lymphocytic leukemia (CLL) diagnostics better to do in cultivated than native cells
Cancer is an age associated disease. In Western countries with people having longer life expectancies than e.g. 100 years ago, this means at present that 1-2% of those individuals are confronted with the diagnosis of leukemia during
Genital melanoma or genital nevus? Molecular answers to a sensitive question
Melanomas arising in the genital region of women are rare although they are the second most common type of cancer in this area. They occur more frequently in women after menopause and tend to be aggressive because
Innovative tools to improve accuracy of long-term biogeochemical measurements in open ocean
The ocean monitoring plans implemented by European countries differ widely for type of observed variables, resolution and accuracy making difficult to obtain an eterogeneous indication about the health of the European seas. This is specially true for
Image Cytometer – NucleoCounter® NC-3000™
The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells.
Reliable molecular imaging of bioluminescence and NIR fluorescence in vivo
In vivo molecular imaging is a powerful tool for non-invasive analysis of cellular and molecular mechanisms. Whole-body optical imaging of small animals is now widely used in preclinical research to explore disease mechanisms, evaluate drug candidates, and
Fluorescence color changes from purple to blue via orange during the solvent evaporative crystallization
The process of crystal formation from solutions is an important subject in fundamental science and also the fabrication of pharmaceuticals, food, polymers and organic solid materials, but remains controversial. In solution crystallization, the formation of crystal nuclei
Substituent-dependent reaction in mechanofluorochromism
Fluorescence switching in response to external stimuli, namely mechanofluorochromism, is highly interesting as this phenomenon can potentially be exploited for sensor, memory, and security ink applications, etc. These responses generally depend on structure alteration upon a mechanical
High-level incorporation of silver in gold nanoclusters for detecting hydrogen peroxide and herbicide
Recent advances in synthesizing noble metal nanoclusters (NCs), in particular Au and Ag NCs, offer a promising class of fluorescent probes for constructing optical sensors, which are expected to improve their features in terms of simplicity, photostability
Recent advances in gold nanoparticle-based optic sensor
The ease in preparation and conjugation, stability, biocompatibility, as well as the size-dependent optical properties makes gold nanoparticles (Au NPs) one of the most popular nanomaterials for sensing of analytes of interest in the last decade. We
Sequence of the plant beneficial biocontrol agent Pseudomonas fluorescens DSM 8569
Pseudomonas represents an abundant bacterial group that can live in the soil. In this habitat many organisms like fungi, bacteria, plants or insects live together, often intensively interacting in close communities. The composition of these communities is
Photometric patch electrode captures optical and electrical signals simultaneously in deep brain tissues
When neurons work they signal each other using action potentials, which is a rapid change of membrane potential and underlies the brain function. In most neurons action potentials accompany changes of intracellular concentration of calcium ions. Calcium
Combining a range of different imaging techniques to better understand the roles metals play in biology
Microscopes in various forms have helped identify disease-causing bacteria, miniaturize microprocessors and engineer superior metal alloys. However, in biology some things remain very difficult to see even at the highest levels of magnification. Metal ions (e.g. calcium,
Pollen and vegetative microspores as sensitive systems for ozone
The high ground-level ozone concentrations (higher than 0.1 μl/l per hour, in which there is a characteristic odor) is an biggest danger for human health. In environmental biomonitoring of O3 hazardous concentrations in air or chronic levels
A simple method for the Study of Acid Sensing Ion Channels (ASICs) by fluorescence
The presence of severe acidosis has been demonstrated to be associated to several pathological conditions, such as multiple sclerosis, brain trauma, spinal cord injury and epilepsy as well as in inflammatory pain, to cite the most relevant.
